AUF1/hnRNP D (D6O4F) Rabbit mAb【科瑞奥博】

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AUF1/hnRNP D (D6O4F) Rabbit mAb

货号： 12382S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	12382S
反应种属	Human
来源宿主	Rabbit
应用	W/IP/IHC-P
目标/特异性	AUF1/hnRNP D (D6O4F) Rabbit mAb recognizes endogenous levels of total AUF1 protein.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:50)

性能
供应商	CST
背景	AU-rich element RNA binding protein 1 (AUF1) is also known as heterogeneous ribonucleoprotein D (hnRNP D). AUF1 binds to the AU rich element (ARE) of target mRNA and regulates mRNA decay (1,2). It has a broad range of target genes including IL-1, IL-2, IL-3, Myc, TNF-α, and cyclin D1 (2). Binding of AUF1 to Myc mRNA also affects translation of Myc (3). Recent studies have provided evidence that AUF1 is also involved in the regulation of transcription. AUF1 binds to the promoters of various genes including complement receptor 2 (4), enkephalin (5), and α-fetoprotein (6). AUF1 also binds to the telomerase catalytic subunit Tert promoter and the G-rich telomeric repeat, thus regulating telomere maintenance and normal aging (7,8). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (9,10). All AUF1 isoforms shuttle between the nucleus and cytoplasm (11, 12). These isoforms have distinct localization and bind to different target mRNAs that contribute to the diversity of AUF1 function (2).
存放说明	-20C
计算分子量	37-48

参考图片

Western blot analysis of extracts from various cells using AUF1/hnRNP D (D6O4F) Rabbit mAb.

Western blot方法检测多种细胞系的提取物，使用的抗体为AUF1/hnRNP D (D6O4F) Rabbit mAb兔单抗。

Immunoprecipitation of AUF1 from HT-29 cell extracts using Normal Rabbit IgG #2729 (lane 2) or AUF1/hnRNP D (D6O4F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using AUF1/hnRNP D (D6O4F) Rabbit mAb and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678.

免疫沉淀法检测HT-29细胞提取物中的AUF1，使用抗体为Normal Rabbit IgG #2729 （Lane 2）或AUF1/hnRNP D (D6O4F) Rabbit mAb兔单抗 (lane 3)。Lane 1为10% input对照。 Western blot 检测中使用的是AUF1/hnRNP D (D6O4F) Rabbit mAb兔单抗以及 Mouse Anti-rabbit IgG (构象特异的) (L27A9) mAb鼠单抗 #3678。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using AUF1/hnRNP D (D6O4F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using AUF1/hnRNP D (D6O4F) Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).