| 货号 | 2087S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Axin1 (C76H11) Rabbit mAb detects endogenous levels of total Axin1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).Axin1（轴心抑制蛋白1）和Axin2为多结构域支架蛋白，并负向调控Wnt信号通路。Axin1与APC, GSK-3β, Dvl和 β-catenin相互作用促进GSK-3β调控的磷酸化并随后β-catenin的降解（1,2）。由于Wnt引起的细胞刺激，Axin1被磷酸化的LRP 5/6招募到细胞膜上,这个过程被认为是Wnt信号通路激活的关键（3,4）。除了在Wnt信号上的作用外，Axin1和MEKK1形成复合物，并激活c-jun氨基末端激酶（JNK/SAPK）（5）。Axin2(也称Conductin 或Axil)在小鼠体内功能上能代替Axin1（6）。Axin2自身直接是Wnt信号通路的靶标，并因此通过负向反馈机制参与控制Wnt通路的强度和持续时间（7-9）。 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of extracts from various cell lines using Axin1 (C76H11) Rabbit mAb. 使用Axin1 (C76H11) 兔单抗对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Axin1 (C76H11) Rabbit mAb. 使用Axin1 (C76H11) Rabbit mAb兔单抗对石蜡包埋的人结肠癌组织进行免疫组化分析。 |
