| 货号 | 2151S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Axin2 (76G6) Rabbit mAb detects endogenous levels of total Axin2 protein. It does not cross-react with Axin1. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 95, 98 |
Western blot analysis of total cell lysates from HCT15 and SW620 cells, using Axin2 (76G2) Rabbit mAb. 使用Axin2 (76G2) Rabbit mAb兔单抗对HCT15和SW620细胞全细胞提取物进行western blot分析。 |
