| 货号 | 5606S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/IF-IC |
| 目标/特异性 | BACE (D10E5) Rabbit mAb detects endogenous levels of total BACE protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | β-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE-1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from HeLa cells, untransfected and BACE transfected, and rat brain extracts using BACE (D10E5) Rabbit mAb. Western blot分析HeLa细胞（未转染或BACE转染）和大鼠脑组织提取物，使用的抗体是BACE (D10E5) Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of mouse hippocampus using BACE1 (D10E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
共聚焦免疫荧光分析小鼠海马区，使用的抗体是BACE1 (D10E5) Rabbit mAb 兔单抗 (绿色)。蓝色伪彩= DRAQ5® #4084 (荧光 DNA 染料)。 |
