| 货号 | 9268S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Bad (11E3) Rabbit mAb (IP Preferred) detects endogenous levels of total Bad protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 21-23 |
Western blot analysis of extracts from OVCAR8 (human), Neuro2A (mouse) and H4IIE (rat) cell lines using Bad (11E3) Rabbit mAb (IP Preferred). Western blot 免疫印记。用Bad (11E3) Rabbit mAb 兔单抗 (更适用于IP实验)分析OVCAR8 (人源), Neuro2A (小鼠源) 和H4IIE (大鼠源) 细胞系的细胞提取液。 | |
Immunoprecipitation/western blot analysis of lysates from HeLa cells. Lane 1 contains lysate input (10%), lanes 2-4 were immunoprecipated with non-specific rabbit IgG, Bad (11E3) Rabbit mAb (IP Preferred) #9268, and Bad Antibody #9292, respectively. Western blot analysis was performed using Bad Antibody #9292. 免疫共沉淀/western blot 免疫印记分析HeLa细胞的裂解液。泳道1包含input裂解液(10%)，泳道2-4分别为用非特异性兔IgG，Bad (11E3) Rabbit mAb (更适合于IP实验) #9268 和Bad Antibody #9292所得到的免疫共沉淀结果。Western blot免疫印记分析所用抗体为Bad Antibody #9292。 |
