Bak (D4E4) Rabbit mAb【科瑞奥博】

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Bak (D4E4) Rabbit mAb

货号： 12105S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	12105S
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit
应用	W/IP/IHC-P/IF-IC/F
目标/特异性	Bak (D4E4) Rabbit mAb recognizes endogenous levels of total Bak protein.
使用方法	WB(1:1000) IP (1:100) IHC-P (1:400) F (1:200) IF-IC (1:200)

性能
供应商	CST
背景	Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).
存放说明	-20C
计算分子量	25

参考图片

Confocal immunofluorescent analysis of OVCAR8 cells using Bak (D4E4) Rabbit mAb (green; left) showing colocalization with mitochondria that were labeled with MitoTracker^® Red CMXRos (red; center). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析OVCAR8细胞，使用Bak (D4E4) Rabbit mAb 兔单抗（绿色；左图），MitoTracker^® Red CMXRos（红色；中心）共定位线粒体。蓝色伪彩=DRAQ5^®＃4084（荧光DNA染料）。

Western blot analysis of extracts from various cell lines using Bak (D4E4) Rabbit mAb.

Western blot 检测多种细胞系提取物，使用抗体为Bak (D4E4) Rabbit mAb 兔单抗。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Bak (D4E4) Rabbit mAb.

免疫组织化学分析石蜡包埋的人乳腺癌切片，使用抗体为Bak (D4E4) Rabbit mAb 兔单抗。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Bak (D4E4) Rabbit mAb.

免疫组织化学分析石蜡包埋的人结肠癌切片，使用抗体为Bak (D4E4) Rabbit mAb 兔单抗。

Immunohistochemical analysis of paraffin-embedded mouse prostate using Bak (D4E4) Rabbit mAb.

免疫组织化学分析石蜡包埋的小鼠前列腺切片，使用抗体为Bak (D4E4) Rabbit mAb 兔单抗。

Flow cytometric analysis of SKBR3 cells using Bak (D4E4) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.