| 货号 | 3498S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Bcl-2 (D17C4) Rabbit mAb (Mouse Preferred) detects endogenous levels of total Bcl-2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
Western blot analysis of extracts from various cell lines using Bcl-2 (D17C4) Rabbit mAb (Mouse Preferred). Western blot 免疫印迹。用Bcl-2 (D17C4) Rabbit mAb (Mouse Preferred) 兔单抗检测各种细胞系的细胞提取液。 | |
