| 货号 | 4122S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | Beclin-1 (2A4) Mouse mAb recognizes endogenous levels of total human Beclin-1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Investigators have demonstrated that Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal (7). Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from various cell lines using Beclin-1 (2A4) Mouse mAb. Western blot分析不同细胞系的提取物，使用的抗体是Beclin-1 (2A4) Mouse mAb 鼠单抗。 |
