| 货号 | 2933S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | Bim (C34C5) Rabbit mAb detects endogenous levels of total Bim (EL, L and S isoforms) protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) F (1:400) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1,2). Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS (1). The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity. |
| 存放说明 | -20C |
| 计算分子量 | 12, 15, 23 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Bim (C34C5) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right). 免疫组织化学染色分析石蜡包埋人乳腺癌组织，用对照多肽(左图)或者抗原特异性多肽(右图)处理，所用抗体为Bim (C34C5) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from Raji, A20 and RL-7 cells using Bim (C34C5) Rabbit mAb. Western blot分析Raji、A20和 RL-7 细胞的细胞提取液，所用抗体是Bim (C34C5) Rabbit mAb兔单抗。 | |
Flow cytometric analysis of Raji cells using Bim (C34C5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞术分析 Raji 细胞，使用 Bim (C34C5) Rabbit mAb 兔单抗(蓝色) 和一个非特异的阴性对照抗体。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Bim (C34C5) Rabbit mAb. 免疫组织化学染色分析石蜡包埋人肺癌组织，所用抗体为Bim (C34C5) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using Bim (C34C5) Rabbit mAb. 免疫组织化学染色分析石蜡包埋人淋巴瘤组织，所用抗体为Bim (C34C5) Rabbit mAb 兔单抗。 |
