| 货号 | 9115S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb detects endogenous levels of total Blimp-1/PRDI-BF1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor that is considered a master regulator of terminal B-cell development (1). The human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-β promoter and can inhibit virus-mediated IFN-β production (2). Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1 (1). In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation (3) and in a subset of T-cells (4,5) suggesting it may play a wider role in homeostasis and differentiation (6). Mechanistically, Blimp-1 is thought to act by recruiting chromatin-modifying enzymes including histone deacetylases (7) and methyltransferases (8,9). Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc (10), CIITA (11), Pax5 (12), Spi-B, and Id3 (13). |
| 存放说明 | -20C |
| 计算分子量 | 95-100 |
Western blot analysis of extracts from Karpas 620, SR and H929 cell lines using Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb.Western免疫印迹。用Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb研究Karpas 620, SR 和 H929 细胞系的细胞提取液。 | |
Confocal immunofluorescent analysis of SR (left) and HeLa cells (right) using Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).共聚焦免疫荧光分析SR细胞(左图)和HeLa细胞(右图),所用抗体为Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb(绿色)。Blue pseudocolor = DRAQ5™ (DNA荧光染料)。 |
