| 货号 | 12396S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | BNIP3L/Nix (D4R4B) Rabbit mAb recognizes endogenous levels of total BNIP3L/Nix protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | BCL2/Adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) (1), also termed BNIP3α (2), B5 (3), and Nix (4), is a member of the Bcl-2 family of apoptotic regulators with highest homology to BNIP3. BNIP3L can bind BNIP3, Bcl-xL, and Bcl-2 (1-5). BNIP3L forms homodimers that withstand denaturing by SDS and reducing conditions (5). BNIP3L is a mitochondrial protein and knockout studies suggest that BNIP3L regulates autophagic clearance of damaged mitochondria during erythroid maturation via mitochondrial autophagy (6,7). It has been shown that the expression of BNIP3L is up-regulated during terminal erythroid differentiation (6-8), as well as in tumor cell lines during hypoxia (9-11). BNIP3L directly regulates the elimination of mitochondria through its ability to bind to and recruit important components of the autophagic machinery, including LC3/Atg8 and GABARAP proteins, via its amino-terminal LC3-interacting region (LIR) (12). BNIP3L may also indirectly activate phagophore formation either via the recruitment of autophagy proteins or by binding Bcl-xL, which in turn releases Beclin-1 (13). BNIP3L/Nix also plays a pivotal role in Parkin-mediated mitochondrial autophagy via its ability to mediate the mitochondrial translocation of Parkin (14). Activated BNIP3L can promote the opening of mitochondrial permeability transition pores resulting in mitochondrial depolarization, generation of reactive oxygen species, and induction of necrosis. Due to its involvement in cell death and autophagy, research scientists have implicated BNIP3L in heart disease and cancer (13). |
| 存放说明 | -20C |
| 计算分子量 | 38, 76 |
Western blot analysis of A172 and HeLa cells, untreated (-) or cobalt chloride-treated (100 μM, overnight; +), using BNIP3L/Nix (D4R4B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot分析 A172和HeLa 细胞，未处理(-)或chloride(氯化钴)处理(100 μM, 过夜; +)，使用的抗体是:BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗（上图）和 β-Actin (D6A8) Rabbit mAb 兔单抗#8457(下图)。 | |
Western blot analysis of extracts from various cell lines using BNIP3L/Nix (D4R4B) Rabbit mAb. Western blot 分析不同细胞系的提取物，使用的抗体是BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing DYKDDDDK-tagged full-length human BNIP3L/Nix (hBNIP3L/Nix-DYKDDDDK; +), using BNIP3L/Nix (D4R4B) Rabbit mAb. The BNIP3L/Nix construct was kindly provided by Dr. Reuben Shaw, Salk Institute for Biological Studies, La Jolla, CA. Western blot 分析293T细胞的提取物，对照转染（-）或DYKDDDDK标记的全长人BNIP3L/Nix (hBNIP3L/Nix-DYKDDDDK; +)载体转染。使用的抗体是BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗。BNIP3L/Nix质粒由Dr. Reuben Shaw, Salk Institute for Biological Studies, La Jolla, CA友情提供。 | |
Immunoprecipitation of BNIP3L/Nix from A172 cells, treated with cobalt chloride (100 μM, overnight), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or BNIP3L/Nix (D4R4B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using BNIP3L/Nix (D4R4B) Rabbit mAb. 免疫沉淀分析A172细胞中的BNIP3L/Nix ，氯化钴处理 (100 μM, 过夜)，使用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 （泳道2），BNIP3L/Nix (D4R4B) Rabbit mAb（泳道3）。泳道1是10% input。Western blot 分析用BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of A172 cells, untreated (left) or cobalt chloride-treated (100 μM, 3 hr; right), using BNIP3L/Nix (D4R4B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析A172细胞，未处理（左）或氯化钴处理（100 μM, 3 小时； 右），使用的抗体是NIP3L/Nix (D4R4B) Rabbit mAb 兔单抗（绿色）。蓝色伪彩= DRAQ5® #4084（DNA荧光染料）。 |
