| 货号 | 14814S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11). |
| 存放说明 | -20C |
| 计算分子量 | 86 |
Western blot analysis of extracts from various cell lines using B-Raf (D9T6S) Rabbit mAb. | |
Immunoprecipitation of B-Raf from Malme-3M cell extracts. Lane 1 represents 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is B-Raf (D9T6S) Rabbit mAb. Western blot analysis was performed using B-Raf (D9T6S) Rabbit mAb. A conformation-specific secondary antibody was used to avoid reactivity with IgG. | |
Western blot analysis of extracts from SK-MEL-28 cells, untreated (-) or treated with 17-AAG #8132 (1 μM, 24 hr; +), using B-Raf (D9T6S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
