| 货号 | 5292S |
| 反应种属 | All |
| 来源宿主 | Mouse |
| 应用 | IHC-P/IF-IC/F |
| 使用方法 | IHC-P(1:200) F (1:200) IF-IC (1:1400) |
| 供应商 | CST |
| 背景 | Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure as well as denaturation of double stranded DNA for various immunodetection applications (1-4).卤代核苷酸如嘧啶类似物溴脱氧尿苷(BrdU)对于新生活细胞和组织中的DNA进行标记是有用的。BrdU掺入复制的DNA后代替胸苷，随后使用允许细胞周期中S期标记的细胞特异性单克隆抗体对BrdU进行免疫检测。溴脱氧尿苷脉冲标记细胞或组织后，BrdU (Bu20a)小鼠单克隆抗体可用于检测掺入单链DNA的BrdU。请参阅我们有关标记程序的详细步骤，以及应用于各种免疫检测的双链DNA变性程序(1-4)。 |
| 存放说明 | -20C |
Immunohistochemical analysis of paraffin-embedded HeLa cells, without (left) and with (right) BrdU incorporation, using BrdU (Bu20a) Mouse mAb.免疫组织化学方法检测石蜡包埋Hela细胞，不伴（左侧）或伴有Brdu掺入（右侧），使用的抗体为 BrdU (Bu20a) Mouse mAb。 | |
Confocal immunofluorescent analysis of HeLa cells using BrdU (Bu20a) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测Hela细胞，是由的抗体为BrdU (Bu20a) Mouse mAb ，呈绿色。蓝色伪彩为DNA荧光染料，产品信息为 DRAQ5® #4084 。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or BrdU incorporated for 30 minutes (green), using BrdU (Bu20a) Mouse mAb.流式细胞术检测Jurkat细胞，不伴有（蓝色）或伴有Brdu掺入（绿色），使用的抗体为BrdU (Bu20a) Mouse mAb。 | |
Flow cytometric analysis of Jurkat cells incorporated with BrdU (30 minutes), using BrdU (Bu20a) Mouse mAb versus propidium iodide (DNA content).流式细胞术检测Jurkat细胞，伴有Brdu掺入（30分钟），使用的抗体为BrdU (Bu20a) Mouse mAb，碘化乙啶检测DNA含量作为对照。 |
