| 货号 | 12457S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of the DNA damage response and DNA repair. BRCA1 protein forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for regulation of BRCA1 function. The BRCA1 A complex includes Rap80, BRCC36, Abraxas, MERIT40/NBA1, and BRE/BRCC45 and functions in G2/M phase checkpoint control (reviewed in 1,2). MERIT40 and BRE maintain the stability of both the BRCA1 A complex and the cytoplasmic BRISC complex, which contains BRCC36 and ABRO1 but not BRCA1 (3). Researchers have shown that the expression level of BRE is related to patient survival in breast cancer (4), and it may predict a favorable outcome in acute myeloid leukemia (AML) (5,6). Studies have also shown that BRE is overexpressed in human hepatocellular carcinoma (7) and that overexpression of BRE can cause resistance to apoptotic signaling and promote tumor growth (7,8).乳腺癌易感基因，BRCA1，编码一种E3泛素连接酶，BRCA1通过调控DNA损伤反应和DNA修复维持基因组的稳定性。BRCA1会和其他DNA损伤蛋白结合形成至少三种不同的复合物（BRCA1A,B和C），这种结合对于调控BRCA1的功能是至关重要的。BRCA1-Rap80 复合物 (BRCA1 A复合物)，包括Rap80, BRCC36, BRCC45, Abraxas, 和MERIT40/NBA1,调控G2/M周期检验点（1,2）。MERIT40和BRE能维持BRCA1 A复合物和细胞质BRISC复合物的稳定性，BRISC复合物包括BRCC36 和ABRO1而不包括BRCA1 (3)。研究人员证实了BRE的表达水平与乳腺癌患者的存活率有关（4），在急性骨髓性白血病中(AML)它可能预示着一个一个好的结果（5,6）。研究也指出在人肝癌组织中BRE是过表达的（7），并且BRE的过表达可能会引发抗凋亡通路并促进肿瘤生长（7,8）。 |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from various cell lines using BRE (D8Q1J) Rabbit mAb.使用BRE (D8Q1J)Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of BRE from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or BRE (D8Q1J) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using BRE (D8Q1J) Rabbit mAb. A light chain-specific secondary antibody was used.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或BRE (D8Q1J)Rabbit mAb(lane 3)对HeLa细胞提取物中的BRE进行免疫沉淀实验。Lane1是10%上样量。BRE (D8Q1J)Rabbit mAb也用于western blot分析。 |
