Btk (C82B8) Rabbit mAb
货号：
3533S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 3533S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Btk (C82B8) Rabbit mAb detects endogenous levels of total Btk. This antibody does not cross-react with other unrelated proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Brutons tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity (8). |
| 存放说明 | -20C |
| 计算分子量 | 77 |
参考图片Western blot analysis of extracts from various cell types using Btk (C82B8) Rabbit mAb.Western免疫印迹。用Btk (C82B8) Rabbit mAb研究各种类型细胞的细胞提取液。 |
