| 货号 | 8547S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 目标/特异性 | Btk (D3H5) Rabbit mAb recognizes endogenous levels of total Btk protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:50) F (1:200) |
| 供应商 | CST |
| 背景 | Brutons tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity (8). |
| 存放说明 | -20C |
| 计算分子量 | 77 |
Western blot analysis of extracts from various cell lines using Btk (D3H5) Rabbit mAb.Western免疫印迹。用Btk (D3H5) Rabbit mAb研究各种类型细胞系的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, Ramos(left) or Jurkat (right), using Btk (D3H5) Rabbit mAb.免疫组织化学染色分析石蜡包埋细胞沉淀Ramos(左图) 和 Jurkat (右图)。所用抗体为Btk (D3H5) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma using Btk (D3H5) Rabbit mAb.免疫组织化学染色分析石蜡包埋人B细胞淋巴瘤。所用抗体为Btk (D3H5) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Btk (D3H5) Rabbit mAb. Note staining of inflammatory cells.免疫组织化学染色分析石蜡包埋人结肠癌组织。所用抗体为Btk (D3H5) Rabbit mAb。注意染色的炎性细胞。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Btk (D3H5) Rabbit mAb. Note staining of inflammatory cells.免疫组织化学染色分析石蜡包埋人卵巢癌组织。所用抗体为Btk (D3H5) Rabbit mAb。注意染色的炎性细胞。 | |
Immunohistochemical analysis of paraffin-embedded mouse colon using Btk (D3H5) Rabbit mAb.免疫组织化学染色分析石蜡包埋小鼠结肠。所用抗体为Btk (D3H5) Rabbit mAb。 | |
Human whole blood was fixed, lysed, and permeabilized as per the Cell Signaling Technology Flow Alternate Protocol and stained using Btk (D3H5) Rabbit mAb. Samples were co-stained using CD3-PE and CD19-APC to distinguish T and B cell subpopulations, respectively. B (red) and T (blue) cell population gates were applied to a histogram depicting the mean fluorescence intensity of Btk. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
