| 货号 | 8225S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | CACYBP (D43G11) Rabbit mAb recognizes endogenous levels of total CACYBP protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:100) |
| 供应商 | CST |
| 背景 | Calcyclin-binding protein or Siah-1-interacting protein (CACYBP/SIP) is a component of the ubiquitin E3 ligase complex that also contains Siah1, Skp1, and Ebi (1). CACYBP regulates β-catenin turnover and plays an important role in thymocyte development (2). CACYBP also binds to tubulin and may be involved in cytoskeletal regulation (3,4). It is highly expressed in neurons, and its cellular localization may be regulated by Ca2+ (5,6). Retinoic acid treatment of the neuroblastoma cell line SH-SY5Y induces translocation of CACYBP to the nucleus and seems to be correlated with phosphorylation of CACYBP on serine residues (7). Recent studies also suggest that CACYBP may possess phosphatase activity (8), and that it can bind and dephosphorylate Erk1/2 (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
Western blot analysis of extracts from various cell lines using CACYBP (D43G11) Rabbit mAb.使用CACYBP (D43G11)兔单抗对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using CACYBP (D43G11) Rabbit mAb.使用CACYBP (D43G11) 对石蜡包埋的人淋巴瘤组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CACYBP (D43G11) Rabbit mAb.使用CACYBP (D43G11) 兔单抗对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded colon carcinoma using CACYBP (D43G11) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).使用CACYBP (D43G11) 兔单抗在对照多肽（左）或抗原特异性多肽（右）存在下对石蜡包埋的结肠癌组织进行免疫组化分析。 | |
Flow cytometric analysis of MCF-7 cells using CACYBP (D43G11) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).使用CACYBP (D43G11) 兔单抗（蓝色）与浓度匹配的兔(DA1E) 单抗 IgG XP® Isotype 对照#3900（红色）对MCF-7细胞进行流式细胞分析。 |
