| 货号 | 2679S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | Calnexin (C5C9) Rabbit mAb detects endogenous levels of total calnexin protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. Calnexin was first identified as being involved in the assembly of murine class I histocompatibility molecules (1,2). Calnexin is a calcium-binding protein embedded in the ER membrane that retains the newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (3-5). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (5). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Confocal immunofluorescent analysis of HeLa cells using Calnexin (C5C9) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞使用Calnexin (C5C9) Rabbit mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Western blot analysis of extracts from PANC1, HepG2 and A204 cells using Calnexin (C5C9) Rabbit mAb. 对PANC1、HepG2和A204细胞抽提液使用Calnexin (C5C9) Rabbit mAb兔单抗进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Calnexin (C5C9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的人乳腺癌使用Calnexin (C5C9) Rabbit mAb兔单抗进行免疫组织化学分析，其中左图为加入对照肽，右图为加入抗原特异性肽。 |
