| 货号 | 11991S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC |
| 目标/特异性 | Calumenin (4C6) Mouse mAb recognizes endogenous levels of total calumenin protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Calumenin belongs to the CREC family of proteins that also contains reticulocalbin, ERC-55/TCBP-49/E6BP, Cab45, and crocalbin/CBP-50. These EF-hand proteins localize to the endoplasmic reticulum (ER) and are involved in the secretory pathway in mammalian cells (1). Calumenin exists as two isoforms corresponding to alternativly spliced variants of the CALU gene (2). Calumenin has been shown to localize to the ER via a carboxy terminal HDEF ER retention signal (3), and undergoes secretion after trafficking through the secretory pathway (4). Secreted calumenin plays a role in amyloidosis by interacting with serum amyloid P component (5). Calumenin is also a chaperone for mutants of the cystic fibrosis transmembrane conductance regulator (CFTR) (6). Researchers performing a proteomics analysis of endometrial cancer identified calumenin as a potential target for endometrial carcinoma (7). |
| 存放说明 | -20C |
| 计算分子量 | 47 |
Western blot analysis of extracts from various cell lines using Calumenin (4C6) Mouse mAb. Western blot方法检测多种细胞系的抽提物，使用的抗体为Calumenin (4C6) Mouse mAb. | |
Confocal immunofluorescent analysis of HeLa cells using Calumenin (4C6) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测HeLa细胞，使用的抗体为Calumenin (4C6) Mouse mAb (绿色). 肌动蛋白微丝用DY-554鬼笔环肽标记(红色)。蓝色伪彩色为DNA荧光染料（产品信息为DRAQ5® #4084）。 |
