| 货号 | 4436S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | CaMKII (pan) (D11A10) Rabbit mAb detects endogenous levels of total CaMKII protein. The peptide sequence used as the antigen is 100% conserved between CaMKII-alpha, gamma and delta, and 88% conserved in CaMKII-beta. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca2+/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4). |
| 存放说明 | -20C |
| 计算分子量 | 60, 50 |
Western blot analysis of extracts from mouse and rat brain using CaMKII (pan) (D11A10) Rabbit mAb. Western blot分析大鼠和小鼠大脑提取物，使用的抗体是CaMKII (pan) (D11A10) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or expressing CaMKII-α (lane2), CaMKII-β (lane 3) or CaMKII-γ (lane 4) using CaMKII (pan) (D11A10) Rabbit mAb. Western blot分析空载体转染的293T细胞（列1）或表达CaMKII-α（列2） 、CaMKII-β（列3）、CaMKII-γ（列4）的细胞提取物，使用的抗体是CaMKII (pan) (D11A10) Rabbit mAb 兔单抗。 |
