CaMKII-α (D10C11) Rabbit mAb
货号：
11945S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 11945S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | CaMKII-α (D10C11) Rabbit mAb recognizes endogenous levels of total CaMKII-α protein. The peptide sequence used as the antigen is not conserved in CaMKII-β, -γ, and -δ. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
参考图片Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human CaMKII-α (hCaMKII-α-Myc;+), CaMKII-β (hCaMKII-β-Myc;+), or CaMKII-δ (hCaMKII-δ-Myc;+), and mouse brain using CaMKII-α (D10C11) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). Western blot方法检测293T细胞提取物：空质粒转染（-）或带Myc-标签全长人CaMKII-α (hCaMKII-α-Myc;+)，CaMKII-β (hCaMKII-β-Myc;+)或CaMKII-δ (hCaMKII-δ-Myc;+)载体转染，以及小鼠大脑；使用的抗体是CaMKII-α (D10C11) Rabbit mAb兔单抗（上图）或Myc-Tag (71D10) Rabbit mAb 兔单抗#2278（下图）。 |
