| 货号 | 4790S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Caspase-8 (D35G2) Rabbit mAb detects endogenous levels of total caspase-8, including the p10 subunit of the activated protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. |
| 存放说明 | -20C |
| 计算分子量 | 10, 57 |
Western blot analysis of extracts from HeLa, THP-1, and YB2/0 cell lines using Caspase-8 (D35G2) Rabbit mAb. Western blot 检测HeLa，THP-1和YB2/0细胞系提取物，使用抗体为 Caspase-8 (D35G2) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from CTLL-2 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml, 4 hours), using Caspase-8 (D35G2) Rabbit mAb. Western blot 检测CTLL-2细胞提取物，未处理 (-)或cycloheximide（放线菌酮）(CHX, 10 μg/ml, 过夜)处理，随后用 Human Tumor Necrosis Factor-α (hTNF-α) #8902 （20 ng/ml, 4 hr）处理，用使用抗体为Caspase-8 (D35G2) Rabbit mAb 兔单抗。 | |
Western blot analysis of 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Caspase-8 protein (hCasp8-Myc/DDK; +), full-length mouse Caspase-8 protein (mCasp8; +), or full-length human Caspase-10 protein (hCasp10; +), using Caspase-8 (D35G2) Rabbit mAb. |
