| 货号 | 46379S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Caveolae ("little caves") are 60-80 nm pits representing specialized plasma membrane domains in many cell types. The principal protein component of caveolae is caveolin, a small integral membrane protein composed of three family members, including the widely expressed caveolin-1 and -2, and the muscle-specific caveolin-3 (1). Caveolin proteins are required for caveolae formation and serve as scaffolding proteins for the recruitment of signaling proteins. Research studies in cavelolin-deficient mice implicate cavelolin proteins in several pathologies, including diabetes, cancer, cardiovascular diseases, atherosclerosis, pulmonary disease, and muscular dystrophies (2). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Confocal immunofluorescent analysis of ACHN (left) and LNCaP (right) cells using Cavin-1 (D8C1D) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Cavin-1 (D8C1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of Cavin-1 from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cavin-1 (D8C1D) Rabbit mAb. Western blot was performed using Cavin-1 (D8C1D) Rabbit mAb. A light chain-specific secondary antibody was used. |
