Cbl-b (D3C12) Rabbit mAb
货号：
9498S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 9498S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Cbl-b (D3C12) Rabbit mAb recognizes endogenous levels of total Cbl-b protein. This antibody does not cross-react with c-Cbl and based upon sequence alignment, is not predicted to cross-react with Cbl-c. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Casitas B lineage lymphoma (Cbl) proteins (in mammals these are c-Cbl, Cbl-b, and Cbl-c) are a family of single subunit RING finger protein-ubiquitin E3 ligases that contain multiple protein interaction motifs (1). All Cbl proteins have a highly conserved N-terminal tyrosine kinase-binding (TKB) domain that mediates interactions between Cbl proteins and phosphorylated tyrosine residues on Cbl substrates. C-terminal to the RING finger, Cbl proteins have proline-rich domains that mediate interactions with SH3 domain-containing proteins. Phosphorylated tyrosine residues mediate interactions with SH2 domain-containing proteins such as the p85 subunit of PI3K. These protein-protein interaction motifs allow Cbl family proteins to function as adaptor proteins (2). This adaptor function contributes to the E3-dependent activities of Cbl proteins by targeting specific substrates for ubiquitination and degradation. The adaptor function also contributes to non-E3-dependent activities, such as the recruitment of proteins involved in receptor tyrosine kinase internalization, localization of Cbl proteins to specific subcellular compartments, and activation of discrete signaling pathways (1). |
| 存放说明 | -20C |
| 计算分子量 | 125, 130 |
参考图片Western blot analysis of extracts from various cell lines using Cbl-b (D3C12) Rabbit mAb. Western blot检测多种细胞提取物。 |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human Cbl-b (hCbl-b-Myc/DDK; +) and Myc/DDK-tagged full-length human c-Cbl (hc-Cbl-Myc/DDK; +), using Cbl-b (D3C12) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower). Western blot检测mock transfected (-)或转染有结构性表达Myc/DDK-tagged全长人c-Cbl (hc-Cbl-Myc/DDK; +)的293T细胞，用Cbl-b (D3C12) Rabbit mAb (upper) 或DYKDDDDK Tag Antibody #2368 (lower)检测。 |
