| 货号 | 12199S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | CCR2 (D14H7) Rabbit mAb recognizes endogenous levels of total CCR2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | CCR2 is a member of the “CC-branch” of chemokine G protein-coupled receptors that regulate monocyte chemotaxis and T cell migration/activation and drive inflammation in a number of pathological conditions (1). CCR2 is the receptor for several chemokines including MCP-1, MCP-3, and MCP-4 (2-5). CCR2 transduces signals through increases in intracellular calcium levels. It has two alternative isoforms, CCR2A and CCR2B, differing in their carboxy-terminal tails with CCR2B trafficking more efficiently to the membrane (2,6). CCR2 was originally identified in the THP-1 monocyte cell line, and its expression is decreased following differentiation into macrophages (7). Knockout studies demonstrate that CCR2 is a major regulator of macrophage trafficking (8-10). In addition, research studies have shown that CCR2 functions as an alternative coreceptor with CD4 for infection of some strains of HIV (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
Immunoprecipitation of CCR2 from THP-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CCR2 (D14H7) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CCR2 (D14H7) Rabbit mAb.免疫沉淀分析来源于THP-1细胞提取物的CCR2。使用的抗体是：Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（泳道2）或CCR2 (D14H7) Rabbit mAb（泳道3）。泳道1是10% input。Western blot分析使用的抗体是CCR2 (D14H7) Rabbit mAb。 | |
Western blot analysis of extracts from various cell lines using CCR2 (D14H7) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). HEL cells are reported to be negative for CCR2 expression (2).Western blot分析来自于不同细胞系的提取物。使用的抗体是：CCR2 (D14H7) Rabbit mAb（上图）、GAPDH (D16H11) XP® Rabbit mAb #5174（下图）。HEL细胞不表达CCR2（2）。 |
