货号 | 13355S |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | W/IP/IHC-P/IF-IC/F |
使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:100) IF-IC (1:100) |
供应商 | CST |
背景 | Intercellular cell adhesion molecule-2 (CD102/ICAM-2) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. Like CD54/ICAM-1, CD102/ICAM-2 is a ligand that binds the leukocyte adhesion molecule LFA-1 (leukocyte function-associated antigen-1), which mediates intercellular interactions between immune cells and other cell types (1). |
存放说明 | -20C |
计算分子量 | 45, 55 |
Confocal immunofluorescent analysis of HUVEC (high expression, left) and HeLa cells (low expression, right) using CD102/ICAM-2 (D7P2Q) Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).使用CD102/ICAM-2 (D7P2Q) Rabbit mAb (绿色)对HUVEC (高表达,左) 和HeLa 细胞 (低表达,右)进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Immunoprecipitation of CD102/ICAM-2 from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CD102/ICAM-2 (D7P2Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CD102/ICAM-2 (D7P2Q) Rabbit mAb. A light chain specific secondary antibody was used.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或CD102/ICAM-2 (D7P2Q) Rabbit mAb (lane 3)对Jurkat细胞提取物的CD102/ICAM-2进行免疫沉淀实验。Lane 1是10%上样量。使用CD102/ICAM-2 (D7P2Q) Rabbit mAb进行western blot分析。实验中使用了一个轻链特异性二抗。 | |
Western blot analysis of extracts from various cell lines using CD102/ICAM-2 (D7P2Q) Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). 使用CD102/ICAM-2 (D7P2Q) Rabbit mAb (上)或β-Actin (13E5) Rabbit mAb #4970 (下)对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from Jurkat cells, untreated (lane 1; -), mock treated (lane 2; -), or peptide-N-glycosidase F (PGNaseF)-treated (lane 3; +), using CD102/ICAM-2 (D7P2Q) Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).使用CD102/ICAM-2 (D7P2Q) Rabbit mAb(上)或 β-Actin (13E5) Rabbit mAb #4970 (下)对未处理(lane 1; -),模拟处理(lane 2; -)或peptide-N-glycosidase F (PGNaseF)处理(lane 3; +)的Jurkat细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded Jurkat (high expression, left) or 293T (low expression, right) cell pellets using CD102/ICAM-2 (D7P2Q) Rabbit mAb.使用CD102/ICAM-2 (D7P2Q) Rabbit mAb对石蜡包埋的Jurkat(高表达,左)或293T(低表达,右)进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil using CD102/ICAM-2 (D7P2Q) Rabbit mAb.使用CD102/ICAM-2 (D7P2Q) Rabbit mAb对石蜡包埋的人扁桃体组织进行免疫组化分析。 | |
Flow cytometric analysis of HeLa cells (blue) and HUVEC (green) using CD102/ICAM-2 (D7P2Q) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.使用CD102/ICAM-2 (D7P2Q) Rabbit mAb对HeLa(蓝色)和HUVEC(绿色)进行流式分析。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414用作二抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD102/ICAM-2 (D7P2Q) Rabbit mAb.使用CD102/ICAM-2 (D7P2Q) Rabbit mAb对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD102/ICAM-2 (D7P2Q) Rabbit mAb.使用CD102/ICAM-2 (D7P2Q) Rabbit mAb对石蜡包埋的肺癌组织进行免疫组化分析。 |