货号 | 3528S |
反应种属 | Human |
来源宿主 | Mouse |
应用 | W/IP/IHC-P/IF-IC/F |
目标/特异性 | CD31 (PECAM-1) (89C2) Mouse mAb detects endogenous levels of total CD31 protein. It does not cross-react with other related proteins. |
使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:400) IF-IC (1:3200) |
供应商 | CST |
背景 | CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells and endothelial cells, and modulates cell adhesion, endothelial cell migration and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical stress or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain containing binding partners such as PI3-kinase, SHIP, PLCγ and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). |
存放说明 | -20C |
计算分子量 | 130 |
Western blot analysis of extracts from HUVEC cells using CD31 (PECAM-1) (89C2) Mouse mAb.Western免疫印迹。用CD31 (PECAM-1) (89C2) Mouse mAb研究HUVEC提取液。 | |
Immunohistochemical analysis of paraffin-embedded HUVEC pellets using CD31 (PECAM-1) (89C2) Mouse mAb.免疫组织化学染色分析石蜡包埋HUVEC沉淀。所用抗体为CD31 (PECAM-1) (89C2) Mouse mAb。 | |
Flow cytometric analysis of HeLa cells (red) and HUVEC (blue), using CD31 (PECAM-1) (89C2) Mouse mAb.流式细胞仪分析HeLa 细胞(红色) 和HUVEC (绿色), 所用抗体为CD31 (PECAM-1) (89C2) Mouse mAb。 | |
Immunohistochemical analysis of paraffin-embedded human smooth muscle using CD31 (PECAM-1) (89C2) Mouse mAb.免疫组织化学染色分析石蜡包埋人平滑肌组织。所用抗体为CD31 (PECAM-1) (89C2) Mouse mAb。 | |
Immunohistochemical analysis of paraffin-embedded human capillary hemangioma using CD31 (PECAM-1) (89C2) Mouse mAb.免疫组织化学染色分析石蜡包埋人毛细血管瘤组织。所用抗体为 CD31 (PECAM-1) (89C2) Mouse mAb。 | |
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb.免疫组织化学染色分析石蜡包埋人透明细胞癌组织。所用抗体为 CD31 (PECAM-1) (89C2) Mouse mAb。 | |
Confocal immunofluorescent analysis of HUVEC (left) and HeLa cells (right) using CD31 (PECAM-1) (89C2) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).共聚焦免疫荧光分析HUVEC (左图) 和 HeLa 细胞(右图) 所用抗体为CD31 (PECAM-1) (89C2) Mouse mAb (绿色)。肌动蛋白微丝用DY-554 phalloidin (红色)标记。Blue pseudocolor = DRAQ5™ (DNA 荧光染料)。 |