货号 | 15094S |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | W/IP/IHC-P |
使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) |
供应商 | CST |
背景 | |
存放说明 | -20C |
计算分子量 | 25-30 (membrane bound); 17 (soluble) |
Western blot analysis of extracts from various cell lines and tissues using CD40 Ligand (D5J9Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of indicated amounts of recombinant Human CD40 Ligand (hCD40L) #3583 using CD40 Ligand (D5J9Y) Rabbit mAb. | |
Immunoprecipitation of CD40 ligand from Jurkat cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CD40 Ligand (D5J9Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CD40 Ligand (D5J9Y) Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG heavy and light chains. | |
Immunoprecipitation of CD40 ligand from a HeLa cell lysate spiked with recombinant Human CD40 Ligand (hCD40L) #3583 (10 ng/ml) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CD40 Ligand (D5J9Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CD40 Ligand (D5J9Y) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded Jurkat (left) and Raji (right) cell pellets using CD40 Ligand (D5J9Y) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human T-cell non-Hodgkins lymphoma using CD40 Ligand (D5J9Y) Rabbit mAb. |