| 货号 | 14866S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | CDC20 binds to and activates the APC/C during mitosis and G1 phase of the cell cycle (12). Moreover, CDC20 is necessary for ubiquitin ligase activity of the APC/C. In metaphase MAD2L1 inactivates the CDC20-APC/C complex, while in anaphase this inhibition is lost and CDC20-APC/C degrades its substrates (13). p53 and p21 suppress expression of CDC20 upon genotoxic stresses and ectopic introduction of p53. siRNA mediated knock-down of CDC20 in cancer cells leads to attenuated cell growth and induces G2/M arrest, suggesting that CDC20 is a possible therapeutic target of cancer (14). Organization of neuronal circuits requires presynaptic axonal differentiation and synapse formation. CDC20-APC regulates presynaptic differentiation in post-mitotic neurons by triggering the required degradation of the transcription factor NeuroD2 (15). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of extracts from various cell lines using CDC20 (D6C2Q) Rabbit mAb. | |
Immunoprecipitation of CDC20 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CDC20 (D6C2Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CDC20 (D6C2Q) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human CDC20 protein (hCDC20-Myc/DDK; +) and Myc/DDK-tagged full-length human FZR1 protein (hFZR1-Myc/DDK; +), using CDC20 (D6C2Q) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower). |
