| 货号 | 81693S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | The 55 kDa centrosomal protein (CEP55) is a widely expressed centrosome and midbody-associated protein that regulates cytokinesis, including completion of the final step during cytokinesis known as abscission (1,2). CEP55 activity during abscission is negatively regulated by p53 through Polo-like kinase 1 (3,4). The breast and ovarian cancer DNA repair protein BRCA2 interacts with CEP55 and plays a regulatory role during abscission (5). Research studies demonstrate that CEP55 is also involved in the regulation of Akt signaling, autophagy, and may be a biomarker in human cancer (reviewed in 6). The correlated overexpression of CEP55, the transcription factor FoxM1, and the HELLS helicase is seen in head and neck squamous cell carcinoma (7,8). Additional studies demonstrate that CEP55 expression regulates cell proliferation in gastric carcinoma (9). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with Doxorubicin #5927 (0.5 μM, 24 hr; +) or UV (100 mJ, 6 hr recovery; +), using CEP55 (D1L4H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of CEP55 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CEP55 (D1L4H) Rabbit mAb. Western blot was performed using CEP55 (D1L4H) Rabbit mAb. A light chain-specific secondary antibody was used. | |
Western blot analysis of extracts from various cell lines using CEP55 (D1L4H) Rabbit mAb. |
