| 货号 | 11891S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | CHD8 (D3C1) Rabbit mAb recognizes endogenous levels of total CHD8 protein. This antibody also cross-reacts with a protein of unknown origin at 140 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | CHD8 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by utilizing the energy derived from ATP hydrolysis to alter chromatin architecture (1,2). The nine CHD family members are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). In addition, CHD8 contains three CR (conserved region) domains, a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like domain, two BRK (brahma and kismet) domains, and a DNA-binding domain (2). The chromatin remodeling activity of CHD8 has been shown to be important for the regulation of a wide variety of genes, such as the HOX genes (5) and genes that are driven by β-catenin (6), p53 (7), estrogen receptor (8), or androgen receptor (9). CHD8 can also interact with the insulator binding protein CTCF and is required for CTCF insulator activity at multiple gene loci (10). |
| 存放说明 | -20C |
| 计算分子量 | 290 |
Western blot analysis of extracts from OVCAR8 and SR cells using CHD8 (D3C1) Rabbit mAb.Western blot方法检测多种细胞系的提取物，使用的抗体为CHD8 (D3C1) Rabbit mAb。 | |
Immunoprecipitation of CHD8 from SH-SY5Y cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CHD8 (D3C1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CHD8 (D3C1) Rabbit mAb.从 SH-SY5Y细胞提取物中免疫沉淀CHD8，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或CHD8 (D3C1) Rabbit mAb (lane 3)。Lane 1为10% input。 使用CHD8 (D3C1) Rabbit mAb进行Western blot检测。 |
