| 货号 | 5554S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | CHOP (D46F1) Rabbit mAb detects endogenous levels of total CHOP protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Western blot analysis of extracts from C2C12 cells, untreated or tunicamycin-treated (2 μg/ml, 8 hr), using CHOP (D46F1) Rabbit mAb (upper) or β-Actin Antibody #4967 (lower). 对C2C12细胞提取物（未处理或tunicamycin处理，2μg/ml，8小时），使用CHOP (D46F1) Rabbit mAb兔单抗（上图）或β-Actin Antibody #4967（下图）进行Western blot分析。 |
