| 货号 | 81376S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | Iron-sulfur (Fe-S) clusters (ISC) are cofactors for many proteins that display a wide range of biological functions, such as DNA maintenance, transcription, translation, cellular metabolism, electron transport, and oxidative phosphorylation (1). While structurally simple, the synthesis and insertion of ISC into Fe-S proteins are complex processes that involve many different proteins. The cytosolic iron-sulfur assembly component 1 (CIAO1) protein is a key component of the cytosolic ISC assembly machinery that incorporates ISC into cytoplasmic and nuclear Fe-S proteins in eukaryotic cells (1,2). CIAO1, along with MMS19, XPD, FAM96B, and ANT2, comprise a complex that localizes to the mitotic spindle during mitosis, which suggests a role in chromosome segregation (3-6). The CIAO1 protein interacts with Wilms tumor suppressor protein (WT1) and may affect its transactivation activity (7). |
| 存放说明 | -20C |
| 计算分子量 | 41 |
Western blot analysis of extracts from various cell lines using CIAO1 (D1B4G) Rabbit mAb. | |
Immunoprecipitation of CIAO1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CIAO1 (D1B4G) Rabbit mAb. Western blot analysis was performed using CIAO1 (D1B4G) Rabbit mAb. |
