| 货号 | 12304S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | CIN85 (D1A4) Rabbit mAb recognizes endogenous levels of total CIN85 protein. This antibody also detects CIN85 isoform b (CD2BP3) and CIN85 isoform c. This antibody does not cross-react with CMS/CD2AP. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | CIN85 was independently identified as Cbl-interacting protein of 85 kDa (1), Ruk (regulator of ubiquitous kinase) (2), SETA (SH3 domain-containing gene expressed in tumorigenic astrocytes) (3), and SH3KBP1 (SH3 domain kinase binding protein 1) (4). The genes encoding these proteins were isolated from either human (CIN85), rat (Ruk and SETA), or mouse (SH3KBP1) sources and share between 92% and 97% sequence identity, suggesting that they represent homologues of one gene. Differential promoter usage and alternative splicing is thought to occur in a tissue specific and developmentally regulated manner to generate a complex expression pattern of various transcripts and encoded protein isoforms (5). The main isoform in humans, CIN85, contains three N-terminal SH3 domains, a proline-rich region harboring several P-X-X-P motifs that provide recognition sites for SH3 domain-containing proteins, a PEST sequence implicated in CIN85 degradation, and a C-terminal coiled-coil region for oligomerization (1,2,5,6). The other molecular variants of CIN85 are shorter, N-terminally truncated proteins lacking one, two, or all three of the SH3 domains (1,5,6-8). Proteomic screens suggest that CIN85 is phosphorylated at multiple sites and the role of phosphorylation of some of these sites in regulation of intra- and intermolecular interactions of CIN85 cannot be excluded. CIN85 belongs to the CD2AP/CMS family of adaptor proteins and has been shown to interact with signaling molecules such as c-Cbl, Cbl-b, BLNK, p85/PI3K, GRB2, p130 Cas, and endophilins to coordinate the activity of multiple signaling cascades. Indeed, a growing body of evidence suggests that CIN85 is required for the regulation of a variety of cellular processes including vesicle-mediated transport (9-12), signal transduction (13,14), and cytoskeleton remodelling (15). |
| 存放说明 | -20C |
| 计算分子量 | 78, 82 |
Western blot analysis of extracts from various cell lines using CIN85 (D1A4) Rabbit mAb.使用CIN85 (D1A4) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human CIN85 isoform a (hCIN85 Isoform a-Myc/DDK; +), CIN85 isoform b (hCIN85 Isoform b-Myc/DDK; +), and CIN85 isoform c (hCIN85 Isoform c-Myc/DDK; +), using CIN85 (D1A4) Rabbit mAb.使用CIN85 (D1A4) Rabbit mAb对未转染（-）或转染了表达Myc/DDK标记的全长人CIN85亚型a(hCIN85 Isoform a-Myc/DDK; +), CIN85 isoform b (hCIN85 Isoform b-Myc/DDK; +),以及CIN85 isoform c (hCIN85 Isoform c-Myc/DDK; +)的质粒的293T细胞提取物进行western blot实验。 | |
Immunoprecipitation of CIN85 from U-937 cell extracts using either Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CIN85 (D1A4) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot detection was performed using CIN85 (D1A4) Rabbit mAb.使用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或CIN85 (D1A4) Rabbit mAb lane 3)对U-937细胞提取物的CIN85进行免疫沉淀实验。Lane 1 is 10%上样量。使用CIN85 (D1A4) Rabbit mAb进行western blot分析。 |
