| 货号 | 28070S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses. |
| 存放说明 | -20C |
| 计算分子量 | 14-16 |
Confocal immunofluorescent analysis of Neuro-2a (left) and NIH/3T3 (right) cells, using Complexin-1/2 (D8A6E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunoprecipitation of complexin-1/2 from Neuro-2A cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Complexin-1/2 (D8A6E) Rabbit mAb. Western blot analysis was performed using Complexin-1/2 (D8A6E) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human complexin-1 (hCPLX-1-Myc; +) or complexin-2 (hCPLX-2-Myc; +), using Complexin-1/2 (D8A6E) Rabbit mAb (upper), and Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Western blot analysis of extracts from Neuro-2a, NIH/3T3, and mouse brain, using Complexin-1/2 (D8A6E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
