| 货号 | 9444S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The COP9 Signalosome (CSN) is a ubiquitously expressed multiprotein complex that is involved in a vast array of cellular and developmental processes, which is thought to be attributed to its control over the ubiquitin-proteasome pathway. Typically, the CSN is composed of eight highly conserved subunits (CSN1-CSN8), each of which is homologous to one of the eight subunits that form the lid of the 26S proteasome particle, suggesting that these complexes have a common evolutionary ancestor (1). CSN was first identified in Arabidopsis thaliana mutants with a light-grown seedling phenotype when grown in the dark (2-4). The subsequent cloning of the constitutive morphogenesis 9 (cop9) mutant from Arabidopsis thaliana was soon followed by the biochemical purification of the COP9-containing multiprotein complex (4). It is now widely accepted that the CSN directly interacts with cullin-RING ligase (CRL) families of ubiquitin E3 complexes, and that CSN is required for their proper function (5). In addition, CSN may also regulate protein homeostasis through its association with protein kinases and deubiquitinating enzymes. Collectively, these activities position the CSN as a pivotal regulator of the DNA-damage response, cell-cycle control, and gene expression (1). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from various cell lines using COPS5 (D15G6) Rabbit mAb. Western blot检测多种细胞提取物。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMD14 (hPSMD14-Myc/DDK; +) and Myc/DDK-tagged full-length human COPS5 (hCOPS5-Myc/DDK; +), using COPS5 (D15G6) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower). Western blot检测 mock transfected (-)或转染表达Myc/DDK-tagged人全长 PSMD14 (hPSMD14-Myc/DDK; +) 和 Myc/DDK-tagged 人全长COPS5 (hCOPS5-Myc/DDK; +)293T细胞，采用COPS5 (D15G6) Rabbit mAb (upper) 或DYKDDDDK Tag Antibody #2368 (lower)。 |
