| 货号 | 4850S |
| 反应种属 | Human/Rat/Monkey/ZebrafishBovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | COX IV (3E11) Rabbit mAb detects endogenous levels of total COX IV protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:1000) F (1:1600) IF-IC (1:250) IHC-F (1:2000) |
| 供应商 | CST |
| 背景 | Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Deficiency in COX activity has been correlated with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control. |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using COX IV (3E11) Rabbit mAb in the presence of control peptide (left) or Cox IV Blocking Peptide #1034 (right). 人乳腺癌石蜡切片的免疫组织化学试验，所用抗体为COX IV Rabbit mAb兔单抗，左为对照肽段，右为Cox IV blocking肽#1304。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing staining of the mitochondria, using COX IV (3E11) Rabbit mAb. 人结肠癌组织的免疫组织化学试验，未线粒体的染色，所用抗体为(3E11) Rabbit mAb兔单抗. | |
Immunohistochemical analysis of paraffin-embedded H1650 xenograft, using COX IV Rabbit mAb. Note specific staining of human cancer cells. 免疫组化检测H1650移植物石蜡切片，所用抗体为COX IV Rabbit mAb兔单抗。留意人肿瘤细胞的特异染色。 | |
Western blot analysis of extracts from HeLa, Jurkat and COS cell lines, using COX IV (3E11) Rabbit mAb. Western blot检测人 HeLa, Jurkat and COS 细胞 ，所用抗体为 COX IV (3E11) Rabbit mAb兔单抗. | |
Flow cytometric analysis of HeLa cells, using COX IV (3E11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). HeLa细胞的流式细胞分选，蓝色为COX IV (3E11) Rabbit mAb兔单抗 ，红色为非特异性对照抗体。 | |
Immunohistochemical analysis of frozen H1650 xenograft, using Cox IV (3E11) Rabbit mAb. 冰冻H1650 外源移植物的免疫组织化学试验，所用抗体为Cox IV (3E11) Rabbit mAb兔单抗 | |
Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测Hela细胞，所用抗体为COX IV (3E11) Rabbit mAb 兔单抗(绿)，微丝用Alexa Fluor® 555 phalloidin 染色(红色)。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084) |
