| 货号 | 11967S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | Cox IV (4D11-B3-E8) Mouse mAb recognizes endogenous levels of total Cox IV protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays. |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Confocal immunofluorescent analysis of HeLa cells using Cox IV (4D11-B3-E8) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa 细胞，使用抗体为 Cox IV (4D11-B3-E8) Mouse mAb 鼠单抗(绿色)。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Cox IV (4D11-B3-E8) Mouse mAb. 免疫组织化学分析人结肠癌石蜡切片，使用抗体为Cox IV (4D11-B3-E8) Mouse mAb鼠单抗。 |
