| 货号 | 13163S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F/IF-IC |
| 目标/特异性 | CRABP1 (D7F9T) Rabbit mAb recognizes endogenous levels of total CRABP1 protein. This antibody does not cross-react with other intracellular lipid-binding protein family members, CRBP1 and CRBP2. |
| 使用方法 | WB(1:1000) IF-F (1:800) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 15 |
Confocal immunofluorescent analysis of mouse embryonic day 10.5 frontal-nasal mesenchyme (left), and mid- to hindbrain (right) using CRABP1 (D7F9T) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用CRABP1 (D7F9T) Rabbit mAb 兔单抗(绿色)对10.5天小鼠胚胎的额鼻间质(左)和中到后脑(右)进行激光共聚焦免疫荧光分析。肌动蛋白丝用DY-554 phalloidin标记(红色)。蓝色伪彩= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Confocal immunofluorescent anaylsis of SK-N-AS (left), or RKO (right) cells using CRABP1 (D7F9T) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用CRABP1 (D7F9T) Rabbit mAb 兔单抗(绿色) SK-N-AS(左)或RKO(右)细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝用DY-554 phalloidin标记(红色)。蓝色伪彩= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Western blot analysis of extracts from various cell lines and human thyroid tissue using CRABP1 (D7F9T) Rabbit mAb. 使用CRABP1 (D7F9T) Rabbit mAb兔单抗对多种细胞系和人甲状腺提取物进行western blot分析。 | |
Western blot analysis of extracts from SK-N-AS (positive) and RKO (negative) cells using CRABP1 (D7F9T) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). 使用CRABP1 (D7F9T) Rabbit mAb 兔单抗(上) 和 GAPDH (D16H11) XP® Rabbit mAb兔单抗 #5174 (下)对SK-N-AS(阳性)和RKO(阴性)提取物进行western blot分析。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with Myc/DDK-tagged constructs expressing full-length human CRABP1 (hCRABP1-Myc/DDK; +), full-length human CRABP2 (hCRABP2-Myc/DDK; +), full-length human CRBP1 (hCRBP1-Myc/DDK; +), full-length human CRBP2 (hCRBP2-Myc/DDK; +), and full length human FABP4 (hFABP4-Myc/DDK; +), using CRABP1 (D7F9T) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower). 模拟转染(-)或转染了Myc/DDK标记分别表达full-length CRABP1 (hCRABP1-Myc/DDK; +), full-length human CRABP2 (hCRABP2-Myc/DDK; +); full-length human CRBP1 (hCRBP1-Myc/DDK; +), full-length human CRBP2 (hCRBP2-Myc/DDK; +), 和 full-length human FABP4 (hFABP4-Myc/DDK; +)质粒的293T细胞提取物，使用CRABP1 (D5W9A) Rabbit mAb 兔单抗(上) 和DYKDDDDK Tag Antibody #2368 (下)进行western blot分析。 |
