Cre Recombinase (D3U7F) Rabbit mAb【科瑞奥博】

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Cre Recombinase (D3U7F) Rabbit mAb

货号： 12830S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	12830S
反应种属	All
来源宿主	Rabbit
应用	W/IF-IC/F
目标/特异性	Cre Recombinase (D3U7F) Rabbit mAb recognizes transfected levels of total Cre recombinase protein.
使用方法	WB(1:1000) F (1:800) IF-IC (1:300)

性能
供应商	CST
背景
存放说明	-20C
计算分子量	37

参考图片

Confocal immunofluorescent analysis of ER-Cre-MEF cells, untreated (left) or treated with 4OH-Tamoxifen (200 ng/ml; center), and Cre-transfected 293 cells (right), using Cre Recombinase (D3U7F) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye). The ER-Cre-MEF cells were kindly provided by Dr. Mark Bedford (M.D. Anderson Cancer Center, TX).共聚焦免疫荧光分析ER-Cre-MEF细胞，未处理（左）或4OH-Tamoxifen处理（200 ng/ml;中），和Cre转染293细胞（右），使用的抗体是：Cre Recombinase (D3U7F) Rabbit mAb（绿色）。肌动蛋白纤维用DY-554 phalloidin标记（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。ER-Cre-MEF细胞由Dr. Mark Bedford (M.D. Anderson Cancer Center, TX)馈赠。

Western blot analysis of extracts from 293T cells, mock-transfected (-) or transfected with a construct expressing Cre recombinase (+), using Cre Recombinase (D3U7F) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).Western blot检测293T细胞提取物，未转染(-)或转染了表达Cre重组酶 (+)，使用的抗体是Cre Recombinase (D3U7F) Rabbit mAb (上图) 和β-Actin (13E5) Rabbit mAb #4970 (下图)。

Flow cytometric analysis of COS-7 cells, untransfected (blue) or transfected with Cre recombinase (green), using Cre Recombinase (D3U7F) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)₂ Fragment (Alexa Fluor^® 647 Conjugate) #4414 was used as a secondary antibody.流式细胞术分析COS-7细胞，未转染（蓝色）或转染了Cre重组酶（绿色）。使用的抗体是Cre Recombinase (D3U7F) Rabbit mAb。二抗是Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414。