CrkL (32H4) Mouse mAb
货号：
3182S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 3182S |
| 反应种属 | Human/Rat |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | CrkL (32H4) Mouse mAb detects endogenous levels of CrkL protein. It does not cross-react with related proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | CrkL, a 39 kDa adaptor protein, has a key regulatory role in hematopoietic cells. CrkL has one SH2 and two SH3 domains, with 60% homology to CrkII (1). The amino-terminal SH3 domain of CrkL binds proteins such as C3G, SOS, PI3K, c-Abl and BCR/Abl. The SH2 domain of CrkL can bind to tyrosine-phosphorylated proteins such as Cbl, HEF1, CAS and paxillin (2,3). CrkL is involved in various signaling cascades initiated by different cytokines and growth factors. The biological outcomes of the Crk-activated signal transduction include the modulation of cell adhesion, cell migration and immune cell responses (4). CrkL is a prominent substrate of the BCR/Abl oncoprotein in chronic myelogenous leukemia and binds to both BCR/Abl and c-Abl (5). CrkL is prominently and constitutively tyrosine phosphorylated in CML neutrophils and is not phosphorylated in normal neutrophils. Moreover, stimulation of normal neutrophils with cytokines and agonists does not induce tyrosine phosphorylation of this protein (6), indicating that it may be a useful target for therapeutic intervention or as a disease marker. Tyr207 in CrkL is the BCR/Abl phosphorylation site (7). |
| 存放说明 | -20C |
| 计算分子量 | 39 |
参考图片Western blot analysis of extracts from HT29 (lane 1), THP1 (lane 2), C6 (lane 3), SUP-M2 (lane 4) and Jurkat (lane 5) cells, using CrkL (32H4) Mouse mAb.Western免疫印迹。用CrkL (32H4) Mouse mAb研究HT29 (泳道 1), THP1 (泳道 2), C6 (泳道3), SUP-M2 (泳道 4)和Jurkat (泳道5) 细胞的细胞提取液。 |
