| 货号 | 2978S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:400) |
| 供应商 | CST |
| 背景 | Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation (4).细胞周期蛋白依赖性蛋白激酶CDK4和CDK6的活性受三个因素的调控：T-环路磷酸化，周期蛋白伴侣(D-型周期蛋白)的丰度，与CDK抑制剂Cip/Kip 或 INK蛋白家族的关系(1)。失活的三元复合物cyclin D/CDK4 和p27 Kip1需要胞外有丝分裂刺激因子参与p27伴侣的释放和降解，伴随周期蛋白D的水平升高，通过限制点和pRb依赖性的S期进入影响细胞周期的进程(2)。活跃的cyclin D/CDK4复合体靶向视网膜母细胞瘤蛋白以利于磷酸化，促进可以激活G1/S期基因表达的转录因子E2F的释放(3)。生长因子退出后，通过下调蛋白表达和磷酸化依赖性蛋白降解，周期蛋白D的水平下降(4)。 |
| 存放说明 | -20C |
| 计算分子量 | 36 |
Western blot analysis of extracts from MCF7, L929 and C6 cells, using Cyclin D1 (92G2) Rabbit mAb.western blot方法分析MCF7、L929和C6细胞提取物，使用的抗体为Cyclin D1 (92G2) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded H1975 xenograft, using Cyclin D1 (92G2) Rabbit mAb.免疫组织化学检测石蜡包埋H1975细胞移植瘤，使用的抗体为Cyclin D1 (92G2) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cyclin D1 (92G2) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人类结肠癌切片，使用的抗体为Cyclin D1 (92G2) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin D1 (92G2) Rabbit mAb in the presence of control peptide (left) or Cyclin D1 Blocking Peptide #1044 (right).免疫组织化学检测石蜡包埋人类乳腺癌切片，左图为使用对照多肽处理的切片，右图为使用Cyclin D1 Blocking Peptide #1044 处理的切片，检测的抗体为Cyclin D1 (92G2) Rabbit mAb。 | |
Flow cytometric analysis of untreated HT29 cells, using Cyclin D1 (92G2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞术检测未处理的HT29细胞，使用Cyclin D1 (92G2) Rabbit mAb (蓝色)抗体与非特异性阴性对照抗体（红色）进行对比。 | |
Immunohistochemical analysis of paraffin-embedded Apc (min/+) mouse intestine using Cyclin D1 (92G2) Rabbit mAb.使用Cyclin D1 (92G2) Rabbit mAb抗体，采用免疫组织化学方法检测石蜡包埋的Apc(min/+) 小鼠肠组织。 |
