| 货号 | 14217S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | CYP11A1, located in the inner membrane of mitochondria, cooperates with two coenzymes, ferredoxin and ferredoxin reductase, to carry out three successive oxidation-reduction reactions of cholesterol (3-5). In the adrenal cortex, testis, and ovary, CYP11A1 expression is regulated by the cAMP-PKA pathway (6), and the transcription factor SF1/NR5A1 has been shown to play a central role in mediating the cAMP signal on the CYP11A1 promoter within steroidogeneic cells of the adrenal cortex and gonads (7). Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46, XY sex reversal (AICSR), which is a rare disorder that can present as acute adrenal insufficiency in infancy or childhood (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Confocal immunofluorescent analysis of NCI-H295R (left, positive) and SW-13 (right, negative) cells, using CYP11A1 (D8F4F) Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot anlaysis of extracts from NCI-H295R cells, MLTC-1 cells, and various tissues using CYP11A1 (D8F4F) Rabbit mAb. | |
Western blot analysis of extracts from NCI-H295R (positive) and SW-13 (negative) cells using CYP11A1 (D8F4F) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). | |
Immunoprecipitation of CYP11A1 from NCI-H295R cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CYP11A1 (D8F4F) Rabbit mAb. Lane 1 is 10% input. Western blot analysis was performed using CYP11A1 (D8F4F) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CYP11A1 protein (hCYP11A1-Myc/DDK; +), using CYP11A1 (D8F4F) Rabbit mAb. |
