| 货号 | 24840S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Cystatin C (cystatin-3, CST3) belongs to the cystatin type 2 superfamily of cysteine peptidase inhibitors (1). The 146 amino acid cystatin C precursor protein contains a 26 residue, amino-terminal secretory signal sequence. The mature 120 amino acid cystatin C protein contains two disulfide bridges (2,3). Cystatin C is secreted in body fluids and is a marker of kidney (4) and cardiovascular (5) dysfunction. Research studies report of changes in cystatin C levels in the cerebral spinal fluid as well as in specific neuronal cell populations in a number of neurodegenerative diseases (6-8). Interestingly, experimental evidence suggests that cystatin C has protective effects against neurodegeneration, such as inhibition of amyloid-β oligomerization and fibril formation (9,10), induction of autophagy (11), induction of neurogenesis (12), and inhibition of cysteine proteases whose activity has been associated with several neurodegenerative diseases (13). |
| 存放说明 | -20C |
| 计算分子量 | 13 |
Immunoprecipitation of cystatin C from Huh7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cystatin C (D6U3E) Rabbit mAb (lane 3). Western blot analysis was performed using Cystatin C (D6U3E) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Cystatin C (D6U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human cystatin C protein (hCysC-Myc; +), using Cystatin (D6U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
