| 货号 | 12963S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | IP/IF-IC |
| 目标/特异性 | Cytochrome c (6H2.B4) Mouse mAb recognizes endogenous levels of total cytochrome c protein by immunofluorescence. This antibody is not recommended for western blot. |
| 使用方法 | IP(1:100) IF-IC (1:300) |
| 供应商 | CST |
| 背景 | Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3). |
| 存放说明 | -20C |
| 计算分子量 | 14 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine (1 μM) #9953 and Z-VAD (50 μM) for 3 hours (right), using Cytochrome c (6H2.B4) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光分析未处理( -)或经过Staurosporine (1 μM) #9953和Z-VAD (50 μM)处理3小时(右)的HeLa细胞，使用Cytochrome c (6H2.B4) Mouse mAb 鼠单抗(绿色)。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Immunoprecipitation of cytochrome c from RD cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Cytochrome c (6H2.B4) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Cytochrome c (D18C7) Rabbit mAb #11940. 免疫沉淀实验分析RD细胞提取物中的细胞色素C蛋白，Lane1是10%上样量，Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) 或 Cytochrome c (6H2.B4) Mouse mAb 鼠单抗(lane 3)。使用Cytochrome c (D18C7) Rabbit mAb 兔单抗#11940进行western blot分析。 |
