货号 | 11940S |
反应种属 | Human/Mouse/Rat/Monkey |
来源宿主 | Rabbit |
应用 | W/IHC-P |
目标/特异性 | Cytochrome c (D18C7) Rabbit mAb recognizes endogenous levels of total cytochrome c protein. |
使用方法 | WB(1:1000) IHC-P (1:100) |
供应商 | CST |
背景 | Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3). |
存放说明 | -20C |
计算分子量 | 14 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Cytochrome c (D18C7) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组织化学染色分析石蜡包埋人乳腺癌组织。在对照多肽(左图)或者抗原特异性肽(右图)存在下,所用抗体为Cytochrome c (D18C7) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from various cell lines using Cytochrome c (D18C7) Rabbit mAb. Western blot 检测多种细胞系提取物,使用抗体为 Cytochrome c (D18C7) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +). Cells were fractionated into whole cell lysate (WCL), cytoplasm (Cyto), membrane (Mem), and cytoskeletal/nucleus (Nuc). Membrane fraction includes mitochondria. Western blot 检测HeLa细胞提取物,未处理 (-)或Staurosporine #9953 (1 μM, 3 hr; +)处理。细胞分离成整个细胞裂解液(WCL),细胞质(Cyto),膜(Mem)和细胞骨架/核(Nuc)。膜部分包括线粒体。 | |
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using Cytochrome c (D18C7) Rabbit mAb. 免疫组织化学染色分析石蜡包埋小鼠骨骼肌切片,所用抗体为Cytochrome c (D18C7) Rabbit mAb 兔单抗。 |