| 货号 | 12906S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | <p>Disabled homologue 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4) and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is down-regulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).</p>Dab2 (disabled homologue 2)是一种有丝分裂原应激磷酸蛋白。通过与大量蛋白结合，它发挥了多种功能。Dab2通过与蛋白结合，例如Smads 和TGF-β受体（1,2），axin（3），GRB (4),和 Src (5)调节多条信号通路（1,2）。Dab2也通过与clathrin (6), AP2 (7), NPXY-containing cargo (8-10), 和 Myosin VI (11,12)结合作为一种clathrin介导内吞作用的cargo特异性接头。除此以外，Dab2通过直接与整合素结合（13,14）调控细胞黏附。Dab2的不同功能使它协调细胞黏附，细胞运动，膜转运和信号通路。在多种癌症中Dab2表达下调，因此提示了它作为一种抑癌因子的作用（15-17）。Dab2磷酸化会降低它的内吞功能（18）。 |
| 存放说明 | -20C |
| 计算分子量 | 96 |
Confocal immunofluorescent analysis of HeLa (left) and OVCAR3 (right) cells using Dab2 (D7O9T) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Dab2 (D7O9T) Rabbit mAb (绿色)对HeLa(左)和OVCAR3(右)进行激光共聚焦荧光分析。肌动蛋白丝用DY-554鬼笔环肽标记(红色)。蓝色假色= DRAQ5®#4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa, A172, and OVCAR3 cells using Dab2 (D7O9T) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457.使用Dab2 (D7O9T) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457对HeLa，A172和OVCAR3细胞提取物进行western blot分析。 | |
Immunoprecipitation of Dab2 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Dab2 (D7O9T) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Dab2 (D7O9T) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或Dab2 (D7O9T) Rabbit mAb (lane 3)对HeLa细胞的Dab2进行免疫沉淀实验。Lan1是10%上样量。使用Dab2 (D7O9T) Rabbit mAb.进行western blot分析。 |
