| 货号 | 12574S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | DAG Lipase β (D4P7C) Rabbit mAb recognizes endogenous levels of total DAG Lipase β protein. In some tissues, this antibody may detect a 48 kDa protein of unknown origin. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Diacylglycerol (DAG) lipases comprise two enzymes called DAG lipase α and β, which are the products of two related genes (1). DAG lipases are transmembrane proteins composed of a short amino-terminal intracellular domain, four transmembrane domains, and a large carboxy-terminal cytoplasmic domain containing the active site. These enzymes are responsible for the biosynthesis of 2-acylglycerol from diacylglycerol in a calcium-dependent manner (1). One of the major endocannabinoid ligands that activate cannabinoid receptors, 2-arachidonyl glycerol (2-AG), is produced by DAG lipases (2). Research studies suggest that DAG lipase α is the isoform primarily responsible for the central production of 2-AG (3). DAG lipase β has been implicated in studies of 2-AG production at the periphery in specific cell types and pathophysiological contexts, such as in hepatic stellate cells during alcohol induced fatty liver (4). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from various cell lines using DAG Lipase β (D4P7C) Rabbit mAb Rabbit mAb. Western blot方法检测多个细胞系的提取物，使用的抗体为DAG Lipase β (D4P7C) Rabbit mAb Rabbit mAb. | |
Immunoprecipitation of DAG Lipase β from MCF7 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DAG Lipase β (D4P7C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DAG Lipase β (D4P7C) Rabbit mAb. 免疫沉淀方法检测MCF7细胞提取物的DAG脂肪酶 β，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或DAG Lipase β (D4P7C) Rabbit mAb (泳道3).泳道1是加入10%蛋白。Western blot检测使用的抗体为DAG Lipase β (D4P7C) Rabbit mAb. |
