| 货号 | 2306S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F |
| 目标/特异性 | DARPP-32 (19A3) Rabbit mAb detects endogenous levels of totoal DARPP-32 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-F (1:400) |
| 供应商 | CST |
| 背景 | DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype (3). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Western blot analysis of extracts from rat and mouse brain using DARPP-32 (19A3) Rabbit mAb. Western blot分析大鼠和小鼠大脑，使用的抗体是DARPP-32 (19A3)Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of normal rat cerebellum, retina, and ciliary epithelium using DARPP-32 (19A3) Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 共聚焦免疫荧光分析正常大鼠小脑、视网膜和纤毛上皮，使用DARPP-32 (19A3) Rabbit mAb 兔单抗（绿色）和Neurofilament-H (RMdO 20) Mouse mAb 鼠单抗#2836（红色）进行标记。蓝色伪彩=DRAQ5™(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded rat retina using DARPP-32 (19A3) Rabbit mAb. 免疫组化分析石蜡包埋的大鼠视网膜，使用的抗体是DARPP-32 (19A3) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain using DARPP-32 (19A3) Rabbit mAb. 免疫组化分析石蜡包埋的小鼠大脑，使用的抗体是DARPP-32 (19A3) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from rat, mouse brain, wild-type mouse striatum and DARPP-32 knock-out mouse striatum, using DARPP-32 (19A3) Rabbit mAb (wild-type mouse striatum and DARPP-32 knock-out mouse striatum was kindly provided by Dr. Paul Greengard and Helen Bateup, Rockefeller University, New York, NY). Western blot分析大鼠、小鼠大脑及野生型和DARPP-32敲除的小鼠纹状体提取物，使用的抗体是DARPP-32 (19A3) Rabbit mAb 兔单抗。（野生型小鼠纹状体和DARPP-32敲除小鼠纹状体由 洛克菲勒大学（纽约）的Dr. Paul Greengard和Helen Bateup提供。 |
