| 货号 | 4533S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Daxx (25C12) Rabbit mAb detects endogenous levels of total Daxx protein. While Daxx has a calculated MW of 81 kDa, it has been shown to run at an apparent MW of 110 kDa at least in part due to post-translational hyper-phosphorylation (5). |
| 使用方法 | WB(1:1000) IF-IC (1:25) |
| 供应商 | CST |
| 背景 | Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (1). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (2,3). Nuclear interactions have since been observed with CENP-C (4), Pax3 (5), DNA methyltransferase I (6) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4 and Dek (7). Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (5). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets including NF-κB and E2F-1 (8). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of extracts from K562 (human), A20 (mouse) and PC12 (rat) cell lines using Daxx (25C12) Rabbit mAb. Western blot 检测 K562 （人）、A20（小鼠）和PC12（大鼠）细胞系提取物，使用抗体为Daxx (25C12) Rabbit mAb兔单抗。 | |
